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sypro orange protein gel stain  (Bio-Rad)


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    Structured Review

    Bio-Rad sypro orange protein gel stain
    Sypro Orange Protein Gel Stain, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 535 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sypro+orange+protein+gel+stain/SYPRO+Ruby+Protein+Gel+Stain/bio_rxiv__2025__11__03__686356-233-16-31
    Average 94 stars, based on 535 article reviews
    sypro orange protein gel stain - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Staining:

    Article Title: SYPRO orange and SYPRO red protein gel stains: one-step fluorescent staining of denaturing gels for detection of nanogram levels of protein.
    Article Snippet: and purity of polypeptides.. The methods chosen for We have developed two new fluorescent dyes, SYdetection of proteins after electrophoresis may dePRO Orange protein gel stain and SYPRO Red propend upon the amount and type of proteins in the tein gel stain, to detect proteins in electrophoretic gel, as well as subsequent use of the identified polygels.. Stained protein bands can be excited by ultravipeptide(s), and requirements for quantitation or storolet light at Ç300 nm, or at visible wavelengths, with age of information from the gel. excitation maxima of 472 nm for the Orange stain The most commonly used gel stain is the triarylmethand 547 nm for the Red stain.

    Article Title: Structure-based interface engineering methodology in designing a thermostable amylose-forming transglucosylase
    Article Snippet: .. The thermal shift (Δ T m ) between recombinant CT-ASase and its variants was preliminarily screened by DSF using SYPRO orange protein gel stain and C1000 Touch thermal cycler (Bio-Rad). ..

    Article Title: Vulnerability in the breadth evolution of an influenza broadly neutralizing antibody
    Article Snippet: SYPRO Orange Protein Gel Stain (5,000X Concentrate in DMSO) (Thermo Fisher Scientific, cat no. S6650). .. 5ug of each Fab was used per assay in a total volume of 25 μL with SYPRO Orange Protein Gel Stain at 1X in PBS in a Hard-Shell 96-Well PCR Plate (Bio-Rad, cat no. HSP9601). ..

    Article Title: Cell-Free Protein Synthesis
    Article Snippet: .. SYPRO Orange protein gel stain (Bio-Rad). ..

    Article Title: Structure-function analysis of alpha-helix H4 using PSE-4 as a model enzyme representative of class A beta-lactamases.
    Article Snippet: Chromatographic columns were run on a ConSep LC100 apparatus (Millipore, Mississauga, Ont.). .. Fractions were selected as above and enzyme purity was estimated from SDS–PAGE gels stained with SYPRO Orange protein gel stain (Bio-Rad, Mississauga, Ont.) and protein density calculated by using the computer software NIH Image version 1.60 (NIH, USA). .. Enzymes were kept in aliquots at –20°C in 50% glycerol and 300 μg/ml ultrapure bovine serum albumin (NEB, Mississauga, Ont.).

    Silver Staining:

    Article Title: SYPRO orange and SYPRO red protein gel stains: one-step fluorescent staining of denaturing gels for detection of nanogram levels of protein.
    Article Snippet: and purity of polypeptides.. The methods chosen for We have developed two new fluorescent dyes, SYdetection of proteins after electrophoresis may dePRO Orange protein gel stain and SYPRO Red propend upon the amount and type of proteins in the tein gel stain, to detect proteins in electrophoretic gel, as well as subsequent use of the identified polygels.. Stained protein bands can be excited by ultravipeptide(s), and requirements for quantitation or storolet light at Ç300 nm, or at visible wavelengths, with age of information from the gel. excitation maxima of 472 nm for the Orange stain The most commonly used gel stain is the triarylmethand 547 nm for the Red stain.

    Recombinant:

    Article Title: Structure-based interface engineering methodology in designing a thermostable amylose-forming transglucosylase
    Article Snippet: .. The thermal shift (Δ T m ) between recombinant CT-ASase and its variants was preliminarily screened by DSF using SYPRO orange protein gel stain and C1000 Touch thermal cycler (Bio-Rad). ..

    Polymerase Chain Reaction:

    Article Title: Vulnerability in the breadth evolution of an influenza broadly neutralizing antibody
    Article Snippet: SYPRO Orange Protein Gel Stain (5,000X Concentrate in DMSO) (Thermo Fisher Scientific, cat no. S6650). .. 5ug of each Fab was used per assay in a total volume of 25 μL with SYPRO Orange Protein Gel Stain at 1X in PBS in a Hard-Shell 96-Well PCR Plate (Bio-Rad, cat no. HSP9601). ..

    Software:

    Article Title: Structure-function analysis of alpha-helix H4 using PSE-4 as a model enzyme representative of class A beta-lactamases.
    Article Snippet: Chromatographic columns were run on a ConSep LC100 apparatus (Millipore, Mississauga, Ont.). .. Fractions were selected as above and enzyme purity was estimated from SDS–PAGE gels stained with SYPRO Orange protein gel stain (Bio-Rad, Mississauga, Ont.) and protein density calculated by using the computer software NIH Image version 1.60 (NIH, USA). .. Enzymes were kept in aliquots at –20°C in 50% glycerol and 300 μg/ml ultrapure bovine serum albumin (NEB, Mississauga, Ont.).



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    Image Search Results


    ArgD binds CphB in both LAH and PAT conditions. A , the relative levels of ArgD and CphB under PAT and LAH in WT and Δ cphB were determined by immunodetection. Whole-cell lysates were separated on SDS-PAGE and blotted onto a PVDF membrane, and the ArgD and CphB proteins were detected using specific antibodies. The Ponceau staining of the membrane is shown for the loading control. Repetitions of the experiment and their statistical analysis are presented in . B , coimmunopurification of f.ArgD with CphB was performed using the same amounts of PAT- or LAH-grown f.argD/ Δ argD cells. The eluates were separated by SDS-PAGE together with the input lysates, including 50% of the lysate from the PAT-grown cells (PAT 50 ). The SYPRO-stained gel was subsequently blotted to a PVDF membrane, which was probed with specific antibodies against CphB and the FLAG tag of ArgD. The control f.ArgD pull-down prepared from the Δ cphB cells is shown in Ref. . CphB, cyanophycinase; f.ArgD, FLAG-tagged variant of ArgD; LAH, light-activated heterotrophic; PAT, photoautotroph; PVDF, polyvinylidene fluoride.

    Journal: The Journal of Biological Chemistry

    Article Title: Cyanophycinase is required for heterotrophy in cyanobacteria

    doi: 10.1016/j.jbc.2025.110791

    Figure Lengend Snippet: ArgD binds CphB in both LAH and PAT conditions. A , the relative levels of ArgD and CphB under PAT and LAH in WT and Δ cphB were determined by immunodetection. Whole-cell lysates were separated on SDS-PAGE and blotted onto a PVDF membrane, and the ArgD and CphB proteins were detected using specific antibodies. The Ponceau staining of the membrane is shown for the loading control. Repetitions of the experiment and their statistical analysis are presented in . B , coimmunopurification of f.ArgD with CphB was performed using the same amounts of PAT- or LAH-grown f.argD/ Δ argD cells. The eluates were separated by SDS-PAGE together with the input lysates, including 50% of the lysate from the PAT-grown cells (PAT 50 ). The SYPRO-stained gel was subsequently blotted to a PVDF membrane, which was probed with specific antibodies against CphB and the FLAG tag of ArgD. The control f.ArgD pull-down prepared from the Δ cphB cells is shown in Ref. . CphB, cyanophycinase; f.ArgD, FLAG-tagged variant of ArgD; LAH, light-activated heterotrophic; PAT, photoautotroph; PVDF, polyvinylidene fluoride.

    Article Snippet: The proteins were separated, visualized with SYPRO orange protein dye (Lumiprobe ProteOrange, catalog no.: 40210) and transferred onto a polyvinylidene fluoride membrane (Sigma–Aldrich, Immobilon-P, catalog no.: IPVH00010) that was subsequently incubated with primary anti-CphB and anti-ArgD antibodies ( ).

    Techniques: Immunodetection, SDS Page, Membrane, Staining, Control, FLAG-tag, Variant Assay